Myelin is a substance rich in lipids and proteins that forms a layer, the myelin sheath, around only the axon of a neuron and acts as insulation. To detect the myelin sheath of peripheral nerves, the traditional method is to use osmic acid staining to obtain images for morphometrical assessment. However, osmic acid is extremely volatile and highly poisonous. It can fix corneas through vapor fixation which can lead to blindness. Inhalation of osmic acid can also lead to pulmonary edema and subsequent death, even at low exposure levels.
Hito Nerve Myelin Sheath OptimStain™ Kit provides a safe and better alternative to osmic acid staining. The kit is non-toxic and easy to use with simplified procedures. It delivers stable and improved staining quality, with minimal overstains / background and artifacts when used properly.
Hito Nerve Myelin Sheath OptimStain™ Kit has been tested on the frozen and paraffin transverse sections of peripheral nerves from several species of animals and proven to be sensitive for demonstrating the morphological details of myelin sheath. It is a simple solution for your research.
Kit Contents (for 150 slides) Solution-1 240 ml Solution-2 120 ml Solution-3 120 ml Solution-4 20 ml Solution-5 125 ml Solution-6 (Nissl Counterstain in Dropper Bottle) 30 ml Staining Jar 2 User Manual and MSDS 1
Before using Hito Nerve Myelin Sheath OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit):Hito Nerve Myelin Sheath OptimStain™ Kit Manual and MSDS
Cryostat or Microtome
Dry ice, O.C.T. compound, isopentane, ethanol, xylene, double distilled or deionized water
Plastic/glass tubes, slides and coverslips
Staining jars for slides wash
Resinous mounting medium
- Additional Information