Myelin is a substance rich in lipids and proteins that forms a layer, the myelin sheath, around only the axon of a neuron and acts as insulation. To detect the myelin sheath of peripheral nerves, the traditional method is to use osmic acid staining to obtain images for morphometrical assessment. However, osmic acid is extremely volatile and highly poisonous. It can fix corneas through vapor fixation which can lead to blindness. Inhalation of osmic acid can also lead to pulmonary edema and subsequent death, even at low exposure levels.
Hito Luxol Fast Blue GMA OptimStain™ Kit provides a safe and better alternative to osmic acid staining. The kit is designed based on Luxol fast >blue method, it is non-toxic and made in a ready-to-use format. This kit offers high quality, rapid staining of myelin sheath of peripheral nerves on the glycolmethacrylate embedded (GMA) sections, proven to be reliable and sensitive to demonstrate the morphological details of myelin sheath of peripheral nerves. It is a simple solution for your research.
Kit Contents Solution-1 (5xConcentrate) 50 ml Solution-2 (5xConcentrate) 50 ml Solution-3 (Nuclear counter stain) 30 ml Dropper Bottle 1 Staining Jars 3 User Manual and MSDS 1
Before using Hito Luxol Fast Blue GMA OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit):
1. Glycolmethacrylate embedded (GMA) sections on slides
2. ethanol, xylene, double distilled or deionized water
4. Staining jars for slides wash
5. Resinous mounting medium
- Additional Information