Hito Luxol Fast Blue - PAS OptimStain™ Kit is designed based on Luxol fast blue and Periodic acid-Schiff (PAS) method. This kit is made in a ready-to-use format and offers high quality, rapid staining (can be finished in one hour) of myelin/myelinated axons on paraffin sections or frozen sections. It has been proven to be extremely reliable and sensitive to demonstrate the morphological details of myelin fibers.
Myelin is a substance rich in lipids and proteins that forms a layer, the myelin sheath, around only the axon of a neuron and acts as insulation. It is essential for the proper functioning of the nervous system. Demyelination is the loss of the myelin sheath insulating the nerves. This impairs the conduction of signals in the affected nerves, causing impairment in sensation, movement, and cognition. Currently, no cure exists for demyelinating diseases and myelin repair is an active research field. Hito Luxol Hito Luxol Fast Blue - PAS OptimStain™ Kit allows sensitive localization and visualization of the myelin fibers. PAS counterstain provides color contrasts which show morphological features that are particularly significant in the tissue, thus offers a fast and reliable way to determine the extent of demyelination.
Hito Luxol Fast Blue - PAS OptimStain™ Kit has been tested extensively on the brains and spinal cords from several species of animals and it is a simple solution for your research.
Kit Contents Standard Kit Small Kit Solution-1 (LFB Solution 10xConcentrate) 125 ml 60 ml Solution-2 (Li2CO3 Solution 5xConcentrate) 250 ml 120 ml Solution-3 (Periodic Acid Solution) 125 ml 60 ml Solution-4 (Modified Schiff's Solution) 125 ml 60 ml Solution-5 30 ml 15 ml Solution-6 125 ml 60 ml Dropper Bottle 1 1 Staining Jars 6 6 User Manual and MSDS 1 1
Before using Hito Luxol Fast Blue - PAS OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit):Hito Luxol Fast Blue - PAS OptimStain™ Kit Manual and MSDS
Cryostat or Microtome and light microscope
Paraffin embedding equipment (or paraffin sections)
Hito Bouin’s Plus Solution (Cat# HTSH0104)
Dry ice, isopentane, O.C.T. compound (for frozen sections), ethanol, xylene, 4% PFA (Hito Cat# HTSHS0102), double distilled or deionized water
Slides and coverslips
Staining jars for slides wash
Resinous mounting medium
- Additional Information