Hito NeuronMyelinStain™ Kit

Hito NeuronMyelinStain™ Kit is designed based on Luxol fast blue and Cresyl echt violet staining method. This kit is made in a ready-to-use format and offers high quality, rapid staining of myelin/myelinated axons and neurons on paraffin sections or frozen sections. It has been proven to be extremely reliable and sensitive for simultaneously demonstrating the morphological details of myelin fibers and neurons.

Myelin is a substance rich in lipids and proteins that forms a layer, the myelin sheath, around only the axon of a neuron and acts as insulation. It is essential for the proper functioning of the nervous system. Demyelination is the loss of the myelin sheath insulating the nerves. This impairs the conduction of signals in the affected nerves, causing impairment in sensation, movement, and cognition. Currently no cure exists for demyelinating diseases and myelin repair is an active research field. Hito NeuronMyelinStain™ Kit allows sensitive localization and visualization of the myelin fibers, thus offers a fast and reliable way to determine the extent of demyelination.
Hito NeuronMyelinStain™ Kit has been tested extensively on the brains and spinal cord from several species of animals and it is a simple solution for your research.

Kit Contents	Standard Kit	Small Kit
Solution-1 (Luxol Fast Blue Solution 10xConcentrate)	125 ml	50 ml
Solution-2 (Lithium Carbonate Solution 5xConcentrate)	250 ml	100 ml
Solution-3 (Cresyl Echt Violet Solution)	30 ml	15 ml
Dropper Bottle	1	1
Staining Jars	3	2
User Manual and MSDS	1	1
Before using Hito NeuronMyelinStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit):   Cryostat or Microtome and light microscope Paraffin embedding equipment (or paraffin sections) Hito Bouin’s Plus Solution (Cat# HTSH0102) Dry ice, isopentane, O.C.T. compound (for frozen sections), ethanol, xylene, 4% PFA (Hito Cat# HTSHS0102), double distilled or deionized water Slides and coverslips Staining jars for slides wash Resinous mounting medium Hito NeuronMyelinStain Kit Manual and MSDS