DAPI (diamidino-2-phenylindole) is a widely used blue fluorescent nuclear and chromosome counterstain. It fluoresces brightly upon selectively binding to AT-rich regions in double-stranded DNA. Its strong DNA selectivity and high cell membrane permeability allow efficient nuclear staining with minimal cytoplasmic background.

DAPI is a classic counterstain for immunofluorescence microscopy. The excitation and emission wavelengths of the DAPI–DNA complex are approximately 360 nm and 460 nm, respectively, providing clear and reliable visualization.

The Hito DAPI Nuclear Counterstain Solution is a ready-to-use reagent for nucleus counterstaining in immunofluorescence experiments, offering a simple and effective solution for your research.