Von Kossa staining has been used to demonstrate deposits of calcium in tissues since the 19th century. The method was originally developed by von Kossa, and underwent several modifications. The Von Kossa stain is not specific for calcium itself, instead it stains calcium salts like calcium phosphate and calcium carbonate, The silver ions in the staining solution binds with phosphate or carbonate by replacing calcium in a precipitation reaction. The silver salts are then reduced to black metallic silver under strong light illumination. So Von Kossa stain allows indirect visualization of the calcium deposits in tissue samples.
Hito Von Kossa OptimStain™ Kit produces brown to black calcium salts against red cytoplasm and nuclei in just 90 minutes. The developer solution is included in the kit, therefore exposure of the slides to strong light, sunlight, or ultra violet light is not required. This stain kit delivers stable and improved staining quality.
For verification of the staining results, Calcium Positive Control Slides are included in the kit (16-18 days mouse embryo).
Hito Von Kossa OptimStain™ Kit has been tested extensively on the different tissue sections from several species of animals and it is a simple solution for your research.
Von Kossa staining has been used to demonstrate deposits of calcium in tissues since the 19th century. The method was originally developed by von Kossa, and underwent several modifications. The Von Kossa stain is not specific for calcium itself, instead it stains calcium salts like calcium phosphate and calcium carbonate, The silver ions in the staining solution binds with phosphate or carbonate by replacing calcium in a precipitation reaction. The silver salts are then reduced to black metallic silver under strong light illumination. So Von Kossa stain allows indirect visualization of the calcium deposits in tissue samples. Hito Von Kossa OptimStain™ Kit produces brown to black calcium salts against red cytoplasm and nuclei in just 90 minutes. The developer solution is included in the kit, therefore exposure of the slides to strong light, sunlight, or ultra violet light is not required. This stain kit delivers stable and improved staining quality. For verification of the staining results, Calcium Positive Control Slides are included in the kit (16-18 days mouse embryo). Hito Von Kossa OptimStain™ Kit has been tested extensively on the different tissue sections from several species of animals and it is a simple solution for your research.
Kit Contents
Standard Kit
Small Kit
Solution-1
100 ml
50 ml
Solution-2
250 ml
125 ml
Solution-3
250 ml
125 ml
Solution-4
250 ml
125 ml
Solution-5
30 ml
15 ml
Dropping Bottle for Solution-5
1
1
Staining Jar (12 ml)
4
4
Calcium Control Slide (Positive)
2
1
User Manual and MSDS
1
1
Before using Hito Von Kossa OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit): Cryostat or microtome Dry ice, isopentane, O.C.T. compound, ethanol, xylene, 4% PFA (recommend Hito Buffered 4% Paraformaldehyde Solution Cat# HTSHS0102), double distilled or deionized water Staining jars for slides wash Resinous mounting medium Light microscope
The office will be temporarily closed during the weeks of May 29- June 07. All orders and inquiries received during this time will be processed first thing on June 08, 2023.