The reticular fiber silver stain is commonly used to demonstrate reticular fibers. The method developed by Gordon and Sweet made further improvement on the reticulin silver stain. However, these reticulin staining procedures use relatively large volume of staining solutions, are costly, time-consuming, and difficult to achieve consistent results.
Hito Reticulin OptimStain™ PAP-Pen Kit offers a simple solution to these problems. This kit is easy to use with simplified procedures. The users can process slides in small quantities of solutions . The staining step can be controlled slide by slide to achieve optimal staining and differentiation for each slide. This kit delivers stable and improved staining quality, with minimal overstains, background and artifacts when used properly.
Hito Reticulin OptimStain™ PAP-Pen Kit has been tested on the liver, spleen, pancreas, lung and other tissues from several species of animals and proven to be sensitive for demonstrating the morphological details of reticular fibers. It is a simple solution for your research.
Kit Contents for 50 Slides Solution-1 20 ml Solution-2 20 ml Solution-3 20 ml Solution-4A 10 ml Solution-4B 10 ml Solution-4C 10 ml Solution-5 20 ml Solution-6 20 ml Solution-7 20 ml Solution-8 20 ml Hito Aqua Barrier PAP Pen 1 User Manual and MSDS 1
Before using Hito Reticulin OptimStain™ PAP-Pen Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit):
Cryostat or Microtome, Light microscope
Paraffin embedding equipment (or paraffin sections)
Hito Bouin’s Plus Solution (Cat# HTSH0102, for paraffin sections preparation)
Dry ice, isopentane, O.C.T. compound (for frozen sections), 4% PFA (Cat# HTSH0101), ethanol, xylene, double distilled or deionized water
Staining jars for slides wash
Resinous mounting medium
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