The Periodic Acid-Schiff (PAS) stain is used for the visualization of polysaccharides, neutral mucosubstances, basement membranes, glycogen and fungi in tissues. PAS staining can be used to assist in the diagnosis of several medical conditions such as glycogen storage disease, fungal infection, etc. Mayer's hematoxylin can be used to counterstain the nuclei blue.
PAS can also be used as counterstain for Luxol Fast Blue staining. It provides color contrasts which show morphological features that are particularly significant in the tissue, thus offers a fast and reliable way to determine the extent of demyelination.
Hito Periodic Acid Schiff (PAS) OptimStain™ Kit has been tested extensively on the tissues from several species of animals. It is a simple solution for your research.
Solution-1 (Periodic Acid Solution) 125 ml Solution-2 (Schiff's Reagent) 125 ml Solution-3 (Hematoxylin Solution) 40 ml Solution-4 125 ml Dropper Bottle 2 Staining Jars 3 User Manual and MSDS 1
Before using Hito Periodic Acid Schiff (PAS) OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit):Hito Periodic Acid Schiff (PAS) OptimStain™ Kit MSDS
Cryostat or Microtome and light microscope
Paraffin embedding equipment (or paraffin sections)
Hito Bouin’s Plus Solution (Cat# HTSH0102)
Dry ice, isopentane, O.C.T. compound (for frozen sections), ethanol, xylene, 4% PFA (Hito Cat# HTSHS0102), double distilled or deionized water
Slides and coverslips
Staining jars for slides wash
Resinous mounting medium
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