Golgi Cox staining or Golgi-Cox impregnation with golgi stain has been recognized as one of the most elegant and effective procedures for studying the morphology of neurons as well as glia. In recent years the Golgi-Cox staining method remains as a primary technique for visualization of the dendritic branching pattern and dendritic spines, because it allows isolation and visualization of the dendritic arbours from a minor random fraction of the neurons in a certain brain area. Accordingly, Golgi techniques are not only useful for pure anatomical studies, but are also widely used in studies examining behavioral-morphological relationships. In some studies, the Golgi-Cox method has been used to identify and study the autonomic innervations in the heart and the results show that the Golgi-Cox method is an attainable and useful tool to identify and study the morphological characteristics of the autonomic innervations in peripheral tissues. However, the Golgi-Cox staining procedure is time-consuming, it requires usage of vibratome, the yields of stained cells are usually low and the results are often unreliable.
Hito Golgi-Cox OptimStain™ Kit offers a simple solution to these problems. Designed based on the methods described by Glaser and Van der Loos, this kit makes dramatic improvement of the Golgi-Cox technique. The procedures are simplified and the processing time is greatly reduced. Users can use cryostat with temperature set at -19ºC. This kit delivers stable and improved staining quality, with minimal overstains and artifacts when used properly. Standard Kit can be used for 50 mouse brains.
If you do not have a cryostat, processed tissue can be prepared as paraffin sections with microtome as an alternative option.
Hito Golgi-Cox OptimStain™ Kit has been tested extensively on the brains, spinal cords and hearts from several species of animals and proven to be sensitive for demonstrating morphological details of neurons and gila, It is a simple solution for your research.
Kit Contents Standard Kit Small Kit Sample Kit Solution-1A (Stock Solution) 100 ml 50 ml 10 ml Solution-1B (Prepare Solution) 150 ml 75 ml Solution-2 250 ml 125 ml 10 ml Solution-3 500 ml 250 ml 20 ml Solution-4 250 ml 125 ml 20 ml Solution-5 250 ml 125 ml 20 ml Dropper Bottle (30 ml Solution-3) 1 1 1 Staining Jar (12 ml) 2 1 1 Fine Tip Natural Hair Brush 2 1 1 Glass Specimen Transfer Tool 2 1 1 User Manual and MSDS 1 1 1
Before using Hito Golgi-Cox OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit):Hito Golgi-Cox OptimStain™ Kit Manual and MSDS
Cryostat (capable of cutting 80- to 200-µm thick sections at –19°C)
Dry ice, O.C.T. compound, isopentane, ethanol, xylene, double distilled or deionized water
Plastic/glass tubes or vials
Gelatin-coated Slide (recommend Hito Dual-Safe Gelatin-coated Slide Cat# HTHS0102) and coverslips
Staining jars for slides wash
Resinous mounting medium
- Additional Information