Hito CryoMyelinStain™ Kit (Gold phosphate complex Myelin Staining Kit) is made in a ready-to-use format and offers high quality, rapid staining of myelin/myelinated axons on frozen sections (mounted or floating). This kit has many advantages compared to the traditional Luxol fast blue staining method which is time-consuming, requires usage of 40-56°C incubator, and usually has low yields of stained myelin fibers and unreliable results. In addition, the long time high temperature incubation process of the frozen sections may cause sections felling off from the slides. Hito CryoMyelinStain kit offers a simple solution to these problems. The procedures are simplified and the processing time is greatly reduced. Users can use mounted sections or floating sections at room temperature. This kit delivers stable and improved staining quality. It has been proven to be extremely reliable and sensitive for demonstrating the morphological details of myelin fibers.
Myelin is essential for the proper functioning of the nervous system. Demyelination impairs the conduction of signals in the affected nerves, causing impairment in sensation, movement, and cognition. Currently no cure exists for demyelinating diseases and myelin repair is an active research field. Hito CryoMyelinStain™ Kit allows sensitive localization and visualization of the myelin fibers, thus offers a fast and reliable way to determine the extent of demyelination [Sample Images: experimental allergic encephalomyelitis (EAE)].
Hito CryoMyelinStain™ Kit has been tested extensively on the brains and spinal cords from several species of animals and it is a simple solution for your research.
Kit Contents (for 60 slides or 200 sections) Solution-1 50 ml 5 ml Solution-2 3 ml 0.3 ml Solution-3 12 ml 1.2 ml Solution-4 250 ml 25 ml Solution-5 25 ml 2.5 ml Staining Jar 2 2 Hito Aqua Barrier PAP Pen (HTHS0110) 1 / Dropper Bottle 1 1 Fine Tip Natural Hair Brush 1 1 Glass Specimen Transfer Tool 1 1 User Manual and MSDS 1 1
Before using Hito CryoMyelinStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit):Hito CryoMyelinStain™ Kit Manual and MSDS
Cryostat and light microscope
Dry ice, isopentane, O.C.T. compound, ethanol, xylene, 4% PFA (recommend Hito Buffered 4% Paraformaldehyde Solution Cat# HTSHS0102), double distilled or deionized water
Gelatin coated slides and coverslips
Staining jars for slides wash
Resinous mounting medium
- Additional Information