The Bielschowsky's silver staining is commonly used to demonstrate axons, neurofibrillary tangles, nerve fibers and senile plaques. This staining technique improved on the method developed by Fajersztajn, who initially applied the silver mirror reaction to tissue staining. Max Bielschowsky a German neuropathologist, improved on Fajersztajn's method by using a silver nitrate solution to incubate formalin-fixed frozen sections, followed by an ammoniacal silver solution, a reducing solution and a sodium thiosulfate solution to remove the unreduced silver. Early modifications of Bielschowsky method were summarized by Beech and Davenport. Further modifications were made by Garven and Gairns, Sevier and Munger, and Yamamoto-Hirano. However, these Bielschowsky staining procedures use relatively large volume of staining solutions, are costly, time-consuming, and difficult to achieve consistent results.
Hito Bielschowsky OptimStain™ Kit offers a simple solution to these problems. This kit is easy to use with simplified procedures. The users can process slides in large or small quantities without wasting the solutions. The developing step can be controlled slide by slide to achieve optimal staining and differentiation. This kit delivers stable and improved staining quality, with minimal overstains/background and artifacts when used properly.
Hito Bielschowsky OptimStain™ Kit has been tested on the brains, spinal cords and peripheral nerves from several species of animals and proven to be sensitive for demonstrating the morphological details of axons and intracellular neurofibrils. It is a simple solution for your research.
Kit Contents Standard Kit Small Kit for 100 slides for 40 slides Solution-1 50 ml 20 ml Solution-2 50 ml 20 ml Solution-3 4 ml 2 ml Solution-4 250 ml 125 ml Hito Aqua Barrier PAP Pen (HTHS0110) 1 1 Staining Jar 2 1 User Manual and MSDS 1 1
Before using Hito Bielschowsky OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit):Hito Bielschowsky OptimStain™ Kit Manual and MSDS
Cryostat or Microtome
Dry ice, O.C.T. compound, isopentane, ethanol, xylene, double distilled or deionized water
Plastic/glass tubes or vials
Slide (recommend Hito Super-Safe slide Cat# HTHS0101) and coverslips
Staining jars for slides wash
Resinous mounting medium
- Additional Information
Customer Reviews 1 item(s)
- Very good bielschowsky's staining kit
Very simple, quick & easy to use, the reaction is controllable, staining quality is really good, the rapid response technical support is useful.
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