Golgi–Cox method requires impregnation of fresh unfixed brain blocks. However, if PFA-fixed or formaldehyde-fixed tissues have to be used, some special treatments for fixed tissues should be performed to get good results.
1. The animal must be completely perfused with buffer and fixative. Perfusion should be performed within five minutes after death of the animal. Once tissues are removed, transfer them into the fixative as soon as possible. Fixed and sucrose solution treated tissues can be stored at -70°C for a long time... ...Read More