OFFICE CLOSED NOTICE: The office will be temporarily closed during the weeks of March 16 - March 23. All orders and inquiries received during this time will be processed first thing on March 26. Thank you for your business and understanding.
Golgi–Cox method requires impregnation of fresh unfixed brain blocks. However, if PFA-fixed or formaldehyde-fixed tissues have to be used, some special treatments for fixed tissues should be performed to get good results.
1. The animal must be completely perfused with buffer and fixative. Perfusion should be performed within five minutes after death of the animal. Once tissues are removed, transfer them into the fixative as soon as possible. Fixed and sucrose solution treated tissues can be stored at -70°C for a long time... ...
How to make gelatin coated slides for Golgi staining?
Gelatin coated slides can hold Golgi sections on slides and prevent sections falling off. Using Hito Gel-Coat™ Solution for Golgi Staining, everyone can make low-cost gelatin coated slides with high quality like commercial grade products.
Prepare animal for infusion by administering a lethal dose of anesthesia. Monitor it until the point when the animal fails to respond to pinching of the foot.
Do not perfuse with buffer or fixative. Remove the tissue (brain, spinal cord or heart) as soon as possible. Handle with care and avoid damage of the tissue. Large specimens should be sliced with a sharp blade into blocks of approximately 10 mm thickness.
Rinse tissue in double distilled water for 2-3 seconds to remove blood from the surface.
Transfer tissue into the mixed impregnation solution that is at least five times the volume of the tissue and store at room temperature in the dark.
Write your review or experience about using our products, no matter positive or negative. If negative, tell us why you feel unsatisfied. Send emails to email@example.com with your order number and order date. All messages will be posted after reviewed and approved by blog administrator. No personal information will be published unless you request so. If your post is approved and published, you’ll get a 10% coupon code for your next order as a token of our appreciation. We also welcome anyone to share their experience about histology staining in this blog, whether using our products or not. Send your posts to firstname.lastname@example.org. If approved and published, you’ll get a 5% discount coupon code for your new order. English is the preferred language for this blog. Other languages are also welcome.